To stain
with DNA InstaStain™ (Ethidium Bromide) sheets
Note:
Ethidium bromide is a CARCINOGEN wear latex gloves at all times. Do
not touch anything with the gloves that you use to handle the gel or staining
papers.
Place the gel on a piece
of plastic wrap on a flat surface.
Moisten the gel with
a few drops of electrophoresis buffer.
Place a sheet of DNA
InstaStain™ (unprinted side down) on the gel.
Firmly run your fingers
over the entire surface of the DNA InstaStain™ several times.
Place your gel casting
tray on top of the gel and weight it down with a small empty beaker (This
insures that the DNA InstaStain™ maintains good contact with the gel).
Allow the DNA InstaStain™
sheet to sit on the gel for 2-5 minutes.
Remove the DNA InstaStain™
sheet. Transfer the gel to a short wave UV illuminator.
Note: Short wave illumination can cause eye damage.
Close
the protective shield on the illuminator and wear goggles before turning
on the illuminator.